8:00 am Registration & Morning Coffee Networking
Synopsis
- Live Demo in Virtual Exhibition Area
- 1-1 Meetings
9:00 am Opening Remarks
9:10 am FDA Insight: Utilizing 3D Models to Drive Drug Development & Regulatory Acceptance
Synopsis
- Outlining the latest developments to incorporate 3D model data to support translational success
- Addressing the evidence required to advance drug candidates from preclinical 3D studies into phase I clinical trials
- The need to adapt guidelines, define context of use across the model continuum and using 3D systems to support in vivo evidence
9:30 am Human Intestinal Organoids to Study the Gut Endocrine Cells
Synopsis
- Genetic engineering of human intestinal organoids to produce and fluorescently track hormone-producing cells
- Employing these organoids to perform an in-depth analysis of gut endocrine cells, uncovering therapeutic targets for metabolic diseases
9:50 am The Development & Application of 3D Culture Techniques to Construct Models of Human Tissues
Synopsis
- Overview of 3D cell culture technologies
- Impact and importance of 3D cell culture
- Beyond 3D cell culture as a technique, applications to for the bioengineering of human tissues in vitro
- Examples of human tissue constructs (eg. skin, intestine) and applications
- New innovations and developments to further enhancing 3D technology and construction of human tissues in vitro
10:10 am Live Q&A – Ask the Speakers Your Burning Questions
10:30 am Morning Networking Break
- Live Demo in Virtual Exhibition Hall
- 1-1 Meetings
Dedicated Roundtable Discussion Chat Room
3D Bioprinting
- Discussing the benefits of utilizing this technique to model complex physiology with different cell types together
- What steps are required to standardize protocol with bioprinting?
11:00 am Patient-Derived Scaffolds Uncover Breast Cancer Promoting Properties of the Microenvironment
Synopsis
- To model and further understand the influence of specific microenvironments we have developed an experimental platform using cell-free patient-derived scaffolds (PDSs) from primary breast cancers infiltrated with standardized breast cancer cell lines
- Patient-derived scaffolds thus mimics in vivo-like growth conditions and uncovers unique information about the malignancy-inducing properties of tumor microenvironment
- PDSs can potentially be used in cancer drug design and screening
11:20 am Human Blood–Brain Barrier On-a-Chip
Synopsis
- Building models of the BBB that recapitulate physiological phenotypes and provides translatable outputs
- Improving drug delivery by assessing candidate drug barrier penetration and brain exposure
11:40 am Application of MPS in Preclinical Biopharmaceutical Development
Synopsis
- Opportunities and challenges for incorporating MPS in preclinical development of biologics
- Maintaining reproducibility in the face of increasing cellular complexity and confounding factors with appropriate biomarkers
- The incorporation of MPS as an alternative to animal models in preclinical development
12:00 pm Live Q&A – Ask the Speakers Your Burning Questions
12:30 pm Networking Lunch
- Live Demo in Virtual Exhibition Hall
- 1-1 Meetings
Dedicated Roundtable Discussion Chat Room
3D Bioprinting
- Discussing the benefits of utilizing this technique to model complex physiology with different cell types together
- What steps are required to standardize protocol with bioprinting?
1:00 pm Aracari Biosciences Case Study
Synopsis
Aracari’s vascularized microphysiological systems are leading the way in pre-clinical drug testing. Our team of specialists are driven to help accelerate drug discovery
1:20 pm 3D Bioprinting of Functional Human Tissue & Disease Models for Preclinical Studies
Synopsis
- Overview of Tissue Bioprinting Group at NCATS
- Bioprinting of vascularized tissue platform
– Ocular tissue (retinal pigment epithelium on “choroid”): modeling wet form age related macular degeneration
– Skin: modeling atopic dermatitis
– Omentum: modeling ovarian cancer metastasis
– Neurovascular Unit: modeling glioblastoma - Bioprinting of neural circuit: modeling opioid user disorder
- Future direction
– Increasing tissue complexity
– Developing towards 96 well format for a drug screening platform
1:40 pm Aspiration-Assisted Bioprinting
Synopsis
- Tissue spheroids have been increasingly used as building blocks for fabrication of tissues, but their precise bioprinting has been a major limitation
- Demonstrated for the first time that by controlling the location and distance between spheroids we can mediate collective capillary sprouting
- Aspiration-assisted bioprinting has great potential to develop tissues not only for tissue engineering but also for disease modeling and microphysiological systems
2:00 pm Automated Electrical Stimulation & Optical Force Readout of 3D Bioprinted Human Muscle Tissues in Microplates
Synopsis
- Discussing a novel device for automated functional readouts such as force and fatigue of 3D bioprinted human skeletal muscle models in microplates
- Using a 3D bioprinting approach, we have developed a new screening platform for the automated fabrication of functional human skeletal muscle tissue models attached between two posts in microwells, which can be electrically stimulated
- It is a promising new in vitro exercise model to identify drugs regulating muscle force and fatigue
2:20 pm Diabetes on a Chip
Synopsis
- Integrated microphysiological systems for the functional interrogation of insulin sensitivity and glucose homeostasis
- Obesity/Diabetes models using human stem cell-derived tissues
2:40 pm Live Q&A – Ask the Speakers Your Burning Questions
3:10 pm Virtual Speed Networking
Synopsis
Recreating the face-to-face networking in the virtual world. We will pair you up with fellow attendees to break the ice and make new business relationships!
3:40 pm Use of Engineered Cardiac Tissues for Applications in Cardio Oncology
Synopsis
- Overview of Biowire II cardiac tissue engineering platform
- Applications for cardio oncology:
- for predicting cardiotoxic responses to a chemotherapeutic agent
- strategies for developing cardioprotective drugs
4:00 pm Advancements in Human Liver Platforms for ADME Studies
Synopsis
- Advancements in Human Liver Platforms for ADME Studies
- Currently available liver systems and their representative drug metabolism validation datasets
- Desired functionality for liver platforms to improve their applicability for practical use in the pharmaceutical industry
4:20 pm A Simple High-Throughput Approach Identifies Actionable Drug Sensitivities in Patient-Derived Tumor Organoids
Synopsis
- Tumor organoids maintain cell-cell interactions, heterogeneity, microenvironment, and drug response of the sample they originate from. Thus, there is increasing interest in developing tumor organoid models for drug development and personalized medicine applications
- Here we introduce a miniaturized method that allows high-throughput screenings in a format compatible with automation using patient-derived tumor organoids
- Requiring few tumor cells, we routinely test combination of chemotherapies or targeted therapies directly on primary tumors from patients’ biopsies or surgical samples with the aim to personalize treatment
4:40 pm Human-on-a-Chip Systems for Use in Therapeutic Index Determination & Systemic Toxicity in Pre-Clinical Drug Discovery Application
Synopsis
- The utilization of multi-organ human-on-a-chip or body-on-a-chip systems for toxicology and efficacy has been suggested as a solution to the lack of good model systems between the single cell level and animal or human systems
- Application of these systems for ALS, Alzheimer’s, rare diseases, diabetes and cardiac and skeletal muscle mechanistic toxicity will be reviewed
- The development of in vitro PDPK models that are being used to predict in vivo results will also be presented